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WISp39 binds phosphorylated Coronin 1B to regulate Arp2/3 localization and Cofilin-dependent motility.

Authors :
Howell, Michael
Brickner, Howard
Delorme-Walker, Violaine D.
Choi, Justin
Saffin, Jean-Michel
Miller, Daniel
Panopoulos, Andreas
DerMardirossian, Céline
Fotedar, Arun
Margolis, Robert L.
Fotedar, Rati
Source :
Journal of Cell Biology. 3/30/2015, Vol. 208 Issue 7, p961-974. 14p.
Publication Year :
2015

Abstract

We previously identified Waf1 Cip1 stabilizing protein 39 (WISp39) as a binding partner for heat shock protein 90 (Hsp90). We now report that WISp39 has an essential function in the control of directed cell migration, which requires WISp39 interaction with Hsp90. WISp39 knockdown (KD) resulted in the loss of directional motility of mammalian cells and profound changes in cell morphology, including the loss of a single leading edge. WISp39 binds Coronin 1B, known to regulate the Arp2/3 complex and Cofilin at the leading edge. WISp39 preferentially interacts with phosphorylated Coronin 1B, allowing it to complex with Slingshot phosphatase (SSH) to dephosphorylate and activate Cofilin. WISp39 also regulates Arp2/3 complex localization at the leading edge. WISp39 KD-induced morphological changes could be rescued by overexpression of Coronin 1B together with a constitutively active Cofilin mutant. We conclude that WISp39 associates with Hsp90, Coronin 1B, and SSH to regulate Cofilin activation and Arp2/3 complex localization at the leading edge. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00219525
Volume :
208
Issue :
7
Database :
Academic Search Index
Journal :
Journal of Cell Biology
Publication Type :
Academic Journal
Accession number :
102023376
Full Text :
https://doi.org/10.1083/jcb.201410095