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Nitrilase-catalyzed hydrolysis of 3-aminopropionitrile at high concentration with a tandem reaction strategy for shifting the reaction to β-alanine formation.
- Source :
-
Journal of Molecular Catalysis B: Enzymatic . May2015, Vol. 115, p113-118. 6p. - Publication Year :
- 2015
-
Abstract
- Given the importance of β-alanine, the nitrilase BjNIT3397 from Bradyrhizobium japonicum strain USDA110 was examined toward the hydrolysis of 3-aminopropionitrile. It has been found that nitrilase BjNIT3397 effectively hydrolyzed 3-aminopropionitrile with substrate concentration up to 3 M (210 g/L) at the pH 7.3 and temperature 30 °C. With the increase of substrate concentration from 0.6 to 3 M, 3-aminopropanamide was formed and its percentage in the products was increased up to 33%. In order to reduce the formation of 3-aminopropanamide, aspartate ammonia-lyase and fumaric acid were added into the reaction system to consume the byproduct ammonia. As expected, the reaction was shifted toward the formation of β-alanine, resulting in the decrease of 3-aminopropanamide from 33% to 3%. Therefore, a tandem reaction strategy was developed to effectively prevent the formation of 3-aminopropanamide. This might also offer a possibility of producing β-alanine and l -aspartic acid in one process. [ABSTRACT FROM AUTHOR]
- Subjects :
- *NITRILASES
*HYDROLYSIS
*ALANINE
*BRADYRHIZOBIUM japonicum
*FUMARATES
Subjects
Details
- Language :
- English
- ISSN :
- 13811177
- Volume :
- 115
- Database :
- Academic Search Index
- Journal :
- Journal of Molecular Catalysis B: Enzymatic
- Publication Type :
- Academic Journal
- Accession number :
- 102073815
- Full Text :
- https://doi.org/10.1016/j.molcatb.2015.02.007