Back to Search
Start Over
Application of MALDI-TOF-MS and nested SAPD-PCR for discrimination of Oenococcus oeni isolates at the strain level.
- Source :
-
Journal of Wine Research . Jun2015, Vol. 26 Issue 2, p69-80. 12p. 1 Diagram, 3 Charts, 1 Graph. - Publication Year :
- 2015
-
Abstract
- Oenococcus oeniis the most important lactic acid bacterium during vinification and is frequently used as a commercial starter culture for malolactic fermentation. Since different strains exhibit a high heterogeneity concerning wine-related physiological characteristics, reliable methods for their differentiation at the strain level are needed, especially for the development of starter cultures. Microbial identification by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has increased over the last few years. In the present study, we used MALDI-TOF-MS for the differentiation of 11O. oeniisolates originating from 5 different wine-growing regions. Furthermore, these strains were analyzed by a DNA fingerprinting method (nested specifically amplified polymorphic DNA (nSAPD)-PCR). Both methods supplied fast and reproducible results allowing a reliable differentiation of theO. oenistrains studied. MALDI-TOF-MS showed a slightly higher discriminatory ability, since two genetically very similar strains could be separated only by the application of this technique. However, nSAPD-PCR and the subsequent cluster analysis permitted a correlation between genetic similarities and the geographical origin of the strains. [ABSTRACT FROM PUBLISHER]
- Subjects :
- *WINES
*LACTIC acid bacteria
*STRAIN theory (Chemistry)
*DNA
*MALOLACTIC enzyme
Subjects
Details
- Language :
- English
- ISSN :
- 09571264
- Volume :
- 26
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Journal of Wine Research
- Publication Type :
- Academic Journal
- Accession number :
- 102077403
- Full Text :
- https://doi.org/10.1080/09571264.2015.1009015