Back to Search
Start Over
Optimization of Three-Dimensional Imaging on In Vitro Produced Porcine Blastocysts and Chimeras for Stem Cell Testing: A Technology Report.
- Source :
-
Stem Cells & Development . May2015, Vol. 24 Issue 9, p1141-1145. 5p. - Publication Year :
- 2015
-
Abstract
- Differential staining is an immunocytochemical staining that visualizes trophectoderm (TE) and the inner cell mass (ICM) of the blastocysts. It is used to determine the blastocyst quality, but could also be a useful tool to assess the integration site of injected cells into the early embryo. This is relevant for testing of presumed pluripotent stem cells. The gold standard for pluripotent stem cells is to test if the cells are capable of contributing to germline chimeras. Differential staining can be used to evaluate the possibility of chimeric contribution; if the cells are located in the area of the ICM they are likely to contribute to the fetus and if they are located in the area of the TE they are likely to contribute to the fetal membranes. In this article, we optimize on methods for embryo staining and mounting so that the exact location of injected stem cells within preimplantation porcine embryos can be evaluated. [ABSTRACT FROM AUTHOR]
- Subjects :
- *STEM cell treatment
*GERM cells
*BLASTOCYST
*CHIMERISM
*FERTILIZATION in vitro
Subjects
Details
- Language :
- English
- ISSN :
- 15473287
- Volume :
- 24
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Stem Cells & Development
- Publication Type :
- Academic Journal
- Accession number :
- 102105031
- Full Text :
- https://doi.org/10.1089/scd.2014.0503