Back to Search
Start Over
Impact of different ChIP-Seq protocols on DNA integrity and quality of bioinformatics analysis results.
- Source :
-
Briefings in Functional Genomics . Mar2015, Vol. 14 Issue 2, p156-162. 7p. - Publication Year :
- 2015
-
Abstract
- Different ChIP-Seq protocolsmay have a significant impact on the final outcome in terms of quality, number and distribution of called peaks. Sample DNA undergoes a long procedure before the final sequencing step, and damaged DNA can result in excessive mismatches in the alignment with reference genome. In this letter, we present the effect of well-defined modifications (timing of formaldehyde crosslink reversal, brand of the sonicator) of standard ChIP-Seq protocol on parallel samples derived from the same cell line correlating the initial DNA quality control metrics to the final bioinformatics analysis results. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20412649
- Volume :
- 14
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Briefings in Functional Genomics
- Publication Type :
- Academic Journal
- Accession number :
- 102220679
- Full Text :
- https://doi.org/10.1093/bfgp/elu001