A fluorescent histone deacetylase (HDAC) inhibitor for cellular imaging.

Authors :: Fleming, Cassandra L.
Ashton, Trent D.
Nowell, Cameron
Devlin, Mark
Natoli, Anthony
Schreuders, Jeannette
Pfeffer, Frederick M.
Source :: Chemical Communications. 5/7/2015, Vol. 51 Issue 37, p7827-7830. 4p.
Publication Year :: 2015
Abstract: Fluorescence microscopy studies using 4-morpholinoscriptaid (4MS) demonstrated rapid cellular uptake of this scriptaid analogue into the cytoplasm but no nuclear penetration. As 4MS and scriptaid have the same in vitro activity against HDACs and KASUMI-1 cells; 4MS exemplifies a rational approach to subtly modify ‘profluorogenic’ substrates for intracellular studies. [ABSTRACT FROM AUTHOR]