Optimization of multiple PCR reaction system in flue-cured tobacco leaf.

Using the single factor and orthogonal experiments with the flue-cured tobacco varieties of zhongyan 100 as raw material and two SCAR primers to study the multiple PCR reaction system in the inter action between the main ingredients of flue-cured tobacco leaves, the best suitable multiple PCR reaction system was established for roast tobacco including DNA template 45 ng, Mg2+ concentration 2.50 mmol/L, dNTP concentration 0.40 mmol/L and Taq enzyme dosage 4.00 U on the 20 µL system. The stripe of the system is clear and it can shorten the identification time, laying a foundation for using multiple PCR technology to identify flue-cured tobacco varieties. [ABSTRACT FROM AUTHOR]