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Regulation of promoter activity of ferredoxin-dependent glutamate synthase
- Source :
-
Plant Physiology & Biochemistry . Jun2003, Vol. 41 Issue 6/7, p649. 7p. - Publication Year :
- 2003
-
Abstract
- The GLU1 promoter for Fd-glutamate synthase (Fd-GOGAT, EC 1.4.1.7) of Arabidopsis thaliana (ecotype Columbia) confers the expression of the β-glucuronidase (GUS) reporter gene on transgenic tobacco (Nicotiana tabacum L. cv. Xanthi) transformed with the GLU1 promoter-GUS construct. Histochemical analysis reveals that GUS expression is associated with mesophyll and vascular tissue of 14-d-old tobacco seedlings. Red light substitutes for white light and induces a 2-fold increase in the GUS expression associated with mesophyll, veins and vascular tissue. Sucrose also serves as a signal to induce GUS expression in mesophyll and veins of cotyledons. Mature leaves, adapted to the dark for 3 d, conserves the red light- and white light-dependent inductions of GUS activity, while GUS expression is repressed by white light in roots. The mesophyll-located expression of the GLU1 promoter suggests that Fd-glutamate synthase has a function in the photorespiratory ammonium cycling and primary ammonium assimilation. The distinct location of GLU1 promoter expression in the vascular tissue supports the view that Fd-glutamate synthase synthesises glutamate for intracellular transport of glutamine and glutamate. [Copyright &y& Elsevier]
- Subjects :
- *ARABIDOPSIS thaliana
*GENETICS
Subjects
Details
- Language :
- English
- ISSN :
- 09819428
- Volume :
- 41
- Issue :
- 6/7
- Database :
- Academic Search Index
- Journal :
- Plant Physiology & Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 10234306
- Full Text :
- https://doi.org/10.1016/S0981-9428(03)00077-9