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Purification and Characterization of Thermostable Cellulase from Consortium XM70 in Terrestrial Hot Spring with Sugarcane Bagasse.
- Source :
-
Tropical Journal of Pharmaceutical Research . Apr2015, Vol. 14 Issue 4, p591-598. 8p. - Publication Year :
- 2015
-
Abstract
- Purpose: To better understand the sugarcane bagasse (SCB) degradation process and obtain thermostable cellulase from terrestrial hot spring. Methods: Molecular community structure of the newly selected thermophilic bacterial consortium XM70 was determined using the method of the full-length 16S rRNA library-based analysis. The thermostable CMCase was purified with ion-exchange and gel filtration chromatography. Results: Sequence-based identification of species belonging to the genera, Geobacillus, Desulfotomaculum, Bacillus, Exiguobacterium, Paenibacillus and Enterobacter were identified. The maximal activities of carboxymethyl cellulase (CMCase), filter paper cellulase and β-glucosidase of the consortium XM70 were obtained after incubation at 60 ℃ and pH 6.0 (4 days), 80 ℃ and pH 7.0 (2 days) and 70 ℃ and pH 8.0 (4 days), respectively. The yield of reducing sugars in the culture broth achieved 0.11 g.g-1 dry SCB. An extracellular CMCase from consortium XM70 (XM70-CMCase) was purified 7.9-fold to apparent homogeneity with a recovery of 65.41% and its molecular mass was about 31.0 kDa. Maximum CMCase activity of the purified XM70-CMCase was 3.77 U/mg at 70 ℃ and pH 7.0. CMCase activity maintained about its maximal value of 70% after incubation at 80 ℃ for 60 min. Conclusion: Due to its high temperature stability, the purified XM70-CMCase may be useful for industrial application such as biofuel, animal feed industry, paper industry and clarification of fruit juices. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15965996
- Volume :
- 14
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Tropical Journal of Pharmaceutical Research
- Publication Type :
- Academic Journal
- Accession number :
- 102373235
- Full Text :
- https://doi.org/10.4314/tjpr.v14i4.5