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MicroRNA-221 regulates endothelial nitric oxide production and inflammatory response by targeting adiponectin receptor 1.
- Source :
-
Gene . Jul2015, Vol. 565 Issue 2, p246-251. 6p. - Publication Year :
- 2015
-
Abstract
- Adiponectin exerts anti-atherosclerosis property through its 2 receptors (AdipoR1 and AdipoR2). The mechanism regulating the expression of adiponectin receptors is unclear. Bioinformatics analysis showed that miR-221 targeted the 3′-untranslated region (3′UTR) of the AdipoR1 mRNA. The protein level and the mRNA level of AdipoR1 were reduced when miR-221 was expressed in human umbilical vein endothelial cells (HUVECs). Meanwhile, miR-221 repressed the activity of luciferase reporter containing the 3′UTR of AdipoR1. The inhibitory effect of miR-221 was abolished when the miR-221 binding site within the AdipoR1 3′UTR was deleted. Overexpression of miR-221 inhibited adiponectin-stimulated nitric oxide (NO) production in HUVECs. Furthermore, miR-221 abolished the inhibitory effect of adiponectin on NF-kB activation and the expression of adhesion molecules. Altogether, these results indicated that miR-221 targets AdipoR1 to regulate endothelial inflammatory response. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MICRORNA
*ENDOTHELIAL cells
*NITRIC oxide
*ADIPONECTIN
*NF-kappa B
Subjects
Details
- Language :
- English
- ISSN :
- 03781119
- Volume :
- 565
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Gene
- Publication Type :
- Academic Journal
- Accession number :
- 102493634
- Full Text :
- https://doi.org/10.1016/j.gene.2015.04.014