Back to Search
Start Over
Isolation and Screening of Broad-spectrum Antibacterial Substance-producing Lactobacillus Strains.
- Source :
-
Agricultural Biotechnology (2164-4993) . Feb2014, Vol. 3 Issue 1, p31-37. 4p. - Publication Year :
- 2014
-
Abstract
- [Objective]This study aimed to screen broad-spectrum antibacterial substance-producing Lactobacillus strains and provide scientific reference for food preservation. [Method] Using homemade pickles as experimental materials, Lactobacillus strains were preliminarily isolated with BCP culture medium containing bromocresol purple; Lactobacillus supernatant was prepared using MRS medium containing CaCO3; subsequently, Lactobacillus strains with antibacterial activity against four indicator bacteria were obtained; after eliminating the interference of acid and hydrogen peroxide, antibacterial active strains were re-screened by thermal stability test and activity persistence test. [Result] After preliminary screening and re-screening, five Lactobacillus strains with antibacterial effects against Escherichia coli, Bacillus subtilis, Staphyloccocus aureus and Saccharomyces cerevisiae were obtained. To be specific,LP-6 strain exhibited significant antibacterial activity after eliminating the interference of acid and hydrogen peroxide. Results of thermal stability test and activity persistence test indicates that the fermentation supernatant of LP-6 strain still had significant antibacterial activity after heating at 121 °C for 15 min and after preservation for three months. [Conclusion] LP-6strain can produce bacteriocin-like antibacterial substances with wide application value in food industry. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 21644993
- Volume :
- 3
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Agricultural Biotechnology (2164-4993)
- Publication Type :
- Academic Journal
- Accession number :
- 102746509