Inhibiting Effect of 12 Guangdong Native Heat-clearing Herbs on EB Virus Antigen Expression and Their Cytotoxicity.

[Objective] To explore inhibiting effect of 12 heat-clearing and detoxicating drugs originated in Guangdong on the expression of EBV viral capsid antigen in cells in vitro and the cytotoxic effect of which on B95-8 cell. [Methods] Study the inhibiting effect of 12 traditional Chinese medicines on the expression of capsid antigen in B95-8 cell by indirect immunoenzyme technique Observe the cytotoxic effect of tradition- al Chinese medicines on B95-8 cell cultured under stimulating circumstances by trypan blue exclusion test. [Results] The expression of VCA in B95-8 cells cultured under stimulating circumstances could be significantly inhibited by 12 heat-clearing and detoxicating drugs originated in Guangdong. Most of the medicines were dose-dependent. The dose of corresponding drug increased 1000 times, the 12 traditional Chinese medicines had significant cytotoxic effect on B95-8 cells and their effects were concentration-dependent. [Conclusions] The inhibiting effect of 12 heat-clearing and detoxicating drugs originated in Guangdong on the expression of EBV viral capsid antigen was significant and with high concentrations, they all had cytotoxic effect on B95-8cells. [ABSTRACT FROM AUTHOR]