Frontispiece: Labeling Strategy and Signal Broadening Mechanism of Protein NMR Spectroscopy in Xenopus laevis Oocytes.

NMR Spectroscopy In their Communication on page 8686 ff., C. Li et al., demonstrate that 19F labeling is a good first choice for studying globular and disordered proteins in Xenopus oocytes, especially compared with conventional 15N ‐ or 13C ‐ methyl enrichment. By using 19F labeling, they found that, unlike E. coli cells, the viscosity in oocytes is only about 1.2 times that of water and that inhomogeneous broadening contributes 60 – 70 % to the line width. The labeling strategies and resonance broadening mechanisms in Xenopus oocytes were explored with the goal of expanding the application of this cell type. [ABSTRACT FROM AUTHOR]