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Bupivacaine induces apoptosis through caspase-dependent and -independent pathways in canine mammary tumor cells.
- Source :
-
Research in Veterinary Science . 2015, Vol. 100, p232-238. 7p. - Publication Year :
- 2015
-
Abstract
- Local anesthetics have been reported to induce apoptosis in various cell lines. In this study, we showed that bupivacaine also induced apoptosis in DTK-SME cells, a vimentin+/AE1+/CK7+/HSP27+, tumorigenic, immortalized, canine mammary tumor cell line. Bupivacaine induced apoptosis in DTK-SME cells in a time- and concentration-dependent manner. Apoptosis-associated morphological changes, including cell shrinkage and rounding, chromatin condensation, and formation of apoptotic bodies, were observed in the bupivacaine-treated DTK-SME cells. Apoptosis was further confirmed with annexin V staining, TUNEL staining, and DNA laddering assays. At the molecular level, the activation of caspases-3, -8, and -9 corresponded well to the degree of DNA fragmentation triggered by bupivacaine. We also demonstrated that the pan-caspase inhibitor, z-VAD-fmk, only partially inhibited the apoptosis induced by bupivacaine. Moreover, treated cells increased expression of endonuclease G, a death effector that acts independently of caspases. Our data suggested that bupivacaine-induced apoptosis occurs through both caspasedependent and caspase-independent apoptotic pathways. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BUPIVACAINE
*APOPTOSIS
*CELL lines
*TUMORS
*CHROMATIN
Subjects
Details
- Language :
- English
- ISSN :
- 00345288
- Volume :
- 100
- Database :
- Academic Search Index
- Journal :
- Research in Veterinary Science
- Publication Type :
- Academic Journal
- Accession number :
- 103126682
- Full Text :
- https://doi.org/10.1016/j.rvsc.2015.03.024