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The method of integrated kinetics and its applicability to the exo-glycosidase-catalyzed hydrolysis of p-nitrophenyl glycosides.
- Source :
-
Carbohydrate Research . Aug2015, Vol. 412, p43-49. 7p. - Publication Year :
- 2015
-
Abstract
- In the present work we suggest an efficient method, using the whole time course of the reaction, whereby parameters k cat , K m and product K I for the hydrolysis of a p- nitrophenyl glycoside by an exo -acting glycoside hydrolase can be estimated in a single experiment. Its applicability was demonstrated for three retaining exo -glycoside hydrolases, β-xylosidase from Aspergillus awamori, β-galactosidase from Penicillium sp. and α-galactosidase from Thermotoga maritima (TmGalA). During the analysis of the reaction course catalyzed by the TmGalA enzyme we had observed that a non-enzymatic process, mutarotation of the liberated α- d -galactose, affected the reaction significantly. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00086215
- Volume :
- 412
- Database :
- Academic Search Index
- Journal :
- Carbohydrate Research
- Publication Type :
- Academic Journal
- Accession number :
- 103655001
- Full Text :
- https://doi.org/10.1016/j.carres.2015.03.021