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GANP DNA primase associated with MCM3 and DNA synthesis
- Source :
-
International Congress Series . Aug2003, Vol. 1255, p283. 6p. - Publication Year :
- 2003
-
Abstract
- GC-associated DNA primase (GANP) contains two functionally important regions, the N-terminal RNA/DNA primase and C-terminal MCM3-associated/import domains, which might be involved in DNA replication. Here, we investigated the functions of these regions by transfection studies. In addition to the C-terminal MCM3-binding (Gmap80) region, the RNA/DNA primase region (Gp) possesses an additional MCM3-interaction activity that mediates its translocation between the nucleus and the cytoplasm. Gp with an N-terminal nuclear localization signal sequence (NLS) is present in the nucleus, but Gmap80 with a C-terminal NLS appeared in the cytoplasm. The whole GANP (Ganp) protein is present in both nuclear and cytoplasmic compartments, in a Crm1-dependent manner. Gp associated with MCM3 through the NLS, and MCM3 induced nuclear localization of Ganp. NLS-negative MCM3 does not have such activity. These results suggest that the NLS of MCM3 is involved in nuclear translocation of Ganp. The nuclear-cytoplasm MCM3 translocation was demonstrated in mammalian cells, and over-expression of Ganp, but not of Gp or Gmap80, facilitated this translocation of MCM3 in co-transfectants. Moreover, introduction of Ganp induced DNA synthesis in the transfectants but dominant-negative mutants did not. It is concluded that GANP is translocated from the nucleus to the cytoplasm in association with MCM3 and is involved in DNA synthesis. [Copyright &y& Elsevier]
- Subjects :
- *DNA replication
*RNA
*CYTOPLASM
Subjects
Details
- Language :
- English
- ISSN :
- 05315131
- Volume :
- 1255
- Database :
- Academic Search Index
- Journal :
- International Congress Series
- Publication Type :
- Academic Journal
- Accession number :
- 10366174
- Full Text :
- https://doi.org/10.1016/S0531-5131(03)00917-8