The impact of fenarimol application on the Magnaporthe poae and fungi in the rhizosphere soil of Kentucky bluegrass (Poa pratensis L.).

[Objectives]The objective of this study was to get deeper insight in the effect of fenarimol application on Magnaporthe poae and fungi properties in the M.poae infected soil. [Methods]Based on the 18s rRNA internal transcribed spacer(ITS)gene,two metagenomic libraries from control and fenarimol treated rhizosphere soil were constructed,and deeper sequencing was carried out by Illumina Genome Analyzer(GA)technology. [Results]There were 4 851 438 and 3 037 924 Illumina reads generated from control and fenarimol treated rhizosphere soil samples,which were further clusterd to 28 219 and 21 288 repetitive operational taxonomic unit(OTU). After fenarimol treated,Ascomycota decreased 18% compared with the control, while Basidiomycota increased 32%,and there were no significant changes of Chytridiomycota and Glomeromycot. In addition,there were 7 phyla,152 genera and 200 fungi species that disappeared after fungicide application. [Conclusions]Fenarimol had significant suppression on M.poae and Phoma, Leptosphaeria, Fusariuma, Phialophora communities in rhizosphere soil. [ABSTRACT FROM AUTHOR]