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Programmed Adsorption and Release of Proteins in a Microfluidic Device.
- Source :
-
Science . 7/18/2003, Vol. 301 Issue 5631, p352-354. 3p. 1 Color Photograph, 3 Graphs. - Publication Year :
- 2003
-
Abstract
- A microfluidic device has been developed that can adsorb proteins from solution, hold them with negligible denaturation, and release them on command. The active element in the device is a 4-nanometer-thick polymer film that can be thermally switched between an antifouling hydrophilic state and a protein-adsorbing state that is more hydrophobic. This active polymer has been integrated into a microfluidic hot plate that can be programmed to adsorb and desorb protein monolayers in less than 1 second. The rapid response characteristics of the device can be manipulated for proteomic functions, including preconcentration and separation of soluble proteins on an integrated fluidics chip. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DENATURATION of proteins
*PROTEINS
*POLYMERS
*PROTEOMICS
Subjects
Details
- Language :
- English
- ISSN :
- 00368075
- Volume :
- 301
- Issue :
- 5631
- Database :
- Academic Search Index
- Journal :
- Science
- Publication Type :
- Academic Journal
- Accession number :
- 10422956
- Full Text :
- https://doi.org/10.1126/science.1080759