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Achyranthes japonica exhibits anti-inflammatory effect via NF-κB suppression and HO-1 induction in macrophages.
- Source :
-
Journal of Ethnopharmacology . Oct2012, Vol. 144 Issue 1, p109-117. 9p. - Publication Year :
- 2012
-
Abstract
- Abstract: Ethnopharmacological relevance: The roots of Achyranthes japonica Nakai have been used in traditional herbal medicine for the treatment of edema and arthritis in Korea. Aim of the study: In this study, we investigated the molecular mechanism responsible for anti-inflammatory effects of the aqueous extract of A. japonica roots (AJ) in LPS-stimulated macrophages. Materials and methods: Nitric oxide (NO) production and as inducible nitric oxide synthase (iNOS) expression were examined in TG-elicited peritoneal macrophages and RAW 264.7 cells. Cell viability was monitored by MTT assay. Protein and mRNA expressions were determined by Western blotting and RT-PCR, respectively. The activity of NF-κB and Nrf2 were examined by EMSA, immunocytochemistry or reporter assay. Results: AJ inhibited LPS-induced NO secretion as well as iNOS expression, without affecting cell viability. Furthermore, AJ suppressed LPS-induced NF-κB activation, degradation of IκB-α, phosphorylation of extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38. Further study demonstrated that AJ induced heme oxygenase-1 (HO-1) gene expression via nuclear translocation and transactivation of Nrf2. In addition, the inhibitory effects of AJ on iNOS expression were abrogated by small interfering RNA-mediated knock-down of HO-1. Conclusions: These results suggest that AJ suppresses LPS-induced NO production and iNOS expression in macrophages through the inhibition of IκB/NF-κB and MAPK as well as the Nrf2-mediated HO-1 induction. These findings provide the scientific rationale for anti-inflammatory therapeutic use of A. japonica roots. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ENZYME metabolism
*ANTI-inflammatory agents
*IN vitro studies
*BIOLOGICAL models
*MACROPHAGES
*NITRIC oxide
*PLANT roots
*REVERSE transcriptase polymerase chain reaction
*DESCRIPTIVE statistics
*PLANT extracts
*MICE
*IMMUNOHISTOCHEMISTRY
*MEDICINAL plants
*ALTERNATIVE medicine
*ANIMAL experimentation
*WESTERN immunoblotting
*BIOLOGICAL assay
*PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 03788741
- Volume :
- 144
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 104423757
- Full Text :
- https://doi.org/10.1016/j.jep.2012.08.037