Back to Search
Start Over
Activity of Hyperthermophilic Glycosynthases Is Significantly Enhanced at Acidic pH.
- Source :
-
Biochemistry . 7/22/2003, Vol. 42 Issue 28, p8484. 10p. 3 Black and White Photographs, 2 Diagrams, 3 Charts, 3 Graphs. - Publication Year :
- 2003
-
Abstract
- We have previously shown that the hyperthermophilic glycosynthase from Sulfolobus solfataricus (Ssβ-glyE387G) can promote the synthesis of branched oligosaccharides from activated β-glycosides, at pH 6.5, in the presence of 2 M sodium formate as an external nucleophile. In an effort to increase the synthetic potential of hyperthermophilic glycosynthases, we report a new method to reactivate the Ssβ-glyE387G glycosynthase and two novel mutants in the nucleophile of the β-glycosidases from the hyperthermophilic Archaea Thermosphaera aggregans (Taβ-gly) and Pyrococcus furiosus (CelB). We describe here that, at pH 3.0 and low concentrations of sodium formate buffer, the three hyperthermophilic glycosynthases show k[sub cat] values similar to those of the wild-type enzymes and 17-fold higher than those observed at the usual reactivation conditions in 2 M sodium formate at pH 6.5. Moreover, at acidic pH the three reactivated mutants have wide substrate specificity and improved efficiency in the synthetic reaction. The data reported suggest that the reactivation conditions modify the ionization state of the residue acting as an acid/base catalyst. This new reactivation method can be of general applicability on hyperthermophilic glycosynthases whose intrinsic stability allows their exploitation as synthetic tools at low pH. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MICROBIAL enzymes
*OLIGOSACCHARIDES
*GLYCOSIDES
Subjects
Details
- Language :
- English
- ISSN :
- 00062960
- Volume :
- 42
- Issue :
- 28
- Database :
- Academic Search Index
- Journal :
- Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 10458398
- Full Text :
- https://doi.org/10.1021/bi0345384