An improved method for the large scale preparation of α,α′-trehalose-6-phosphate.

An improved method was developed for the efficient, large-scale synthesis of α,α′-trehalose-6-phosphate starting with α,α′-trehalose. In the sequence, octa-trimethylsilylation of α,α′-trehalose is followed by selective removal of the 6- O -trimethylsilyl protecting group. Reaction of the mono-alcohol with (PhO) 2 POCl is then followed by simultaneous hydrogenolysis and per-desilylation to generate α,α′-trehalose-6-phosphate, free of contamination by inorganic phosphate. [ABSTRACT FROM AUTHOR]