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Amplified electrochemical sensing of lead ion based on DNA-mediated self-assembly-catalyzed polymerization.
- Source :
-
Biosensors & Bioelectronics . Jul2015, Vol. 69, p230-234. 5p. - Publication Year :
- 2015
-
Abstract
- Lead ion (Pb 2+ , a heavy metal ion existed every aspect of life) is one of the most important hazards for environment and human health. Herein we design an in-situ amplified electronic monitoring system for sensitive determination of Pb 2+ on Pb 2+ -specific DNAzyme-modified sensing interface. The assay consists of target-triggered cleavage of Pb 2+ -specific DNAzyme, initiator strand-induced DNA hybridization chain reaction, formation of hemin/G-quadruplex -based DNAzyme and its automatically catalyzed polymerization of aniline monomer along the double-stranded DNA. Experimental results indicated that the catalytic currents of the as-produced polyaniline were linearly dependent on target Pb 2+ concentrations from 0.05 to 50 nM with a detection limit (LOD) of 32 pM. Such a synergistic effect of hybridization chain reaction with DNAzyme-catalyzed polymerization provided a universal platform for sensitive screening of target Pb 2+ , thereby holding great promise for application in practice. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09565663
- Volume :
- 69
- Database :
- Academic Search Index
- Journal :
- Biosensors & Bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 108296084
- Full Text :
- https://doi.org/10.1016/j.bios.2015.02.041