The Effects of α-Tocopherol on Endometrial Uterine Vascularization, Uterine Cervix Oxidative Stress and Proliferation in Female Rats Exposed to Tobacco Smoke.

Purpose: This study aimed toknow whether administration of α-tocopherolable to increase uterine endometrial vascularization, inhibit uterine cervix oxidative stress, and augment cervical proliferation in female rats exposed to tobacco smoke. Material and Methods:Twenty five, 8 weeks old female Wistar rats (Rattusnorvegicus) were divided into 5 groups (n=5 each), including control group (non exposure group); the rats were exposed to tobacco smoke (CS); the rats were exposed to tobacco smoke and treated with α-tocopherol at dose of 100 mg/kg/day (CS + AT1); rats were exposed to tobacco smoke and treated with α-tocopherol at dose of 200mg/kg/day (CS + AT2); rats were exposed to tobacco smoke and treated with α-tocopherol at dose of 400 mg/kg/day (CS + AT3). The expression of endometrial VEGF was observed by immunohistochemical staining using anti-VEGF primary antibody and biotinylated universal secondary antibody. MDA levels were measured colorimetrically. The number of spiralis arterioles and proliferation of uterine cervix epithelial cells were counted histologically withhematoxilin eosin staining. This research has been approved by research ethics committee Faculty of Medicine University of Brawijaya, Malang, Indonesia (Number 477/EC/KEPK-S2- KB/10/2013). Results: Tobacco smoke significantly lowered VEGF level, the number of spiralis arterioles, anduterine cervix epithelial cells compared with control group (P < 0.05). Administration of α-tocopherol in different doses significantly increasethe VEGF level to reachs the level in the control group (P > 0.05). Second and third doses of α-tocopherolsignificantly increased the number of spiralis arterioles compared with CS group (P < 0.05), to reached the similar level in the control group (P > 0.05).Exposure to tobacco smoke in rats can increase uterine cervix MDA level significantly compared to the control (P< 0.05). The α-tocopherol in various doses cansignificantly reduce MDA level compared to the CS (P <0.05), but has not reached the levels of the control group. The number of epithelial cells in α-tocopherol-administered groupwas not significantly different compared with CS group (P > 0.05). Conclusion: Administration of α-tocopherolcan increase the vascularization in endometrial uterine of rats exposed to tobacco smoke. Besides, α-tocopherolinhibit uterine cervix oxidative stress, but can't induces the proliferation of uterine cervix epithelial cells. [ABSTRACT FROM AUTHOR]