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Effect of kojic acid-grafted-chitosan oligosaccharides as a novel antibacterial agent on cell membrane of gram-positive and gram-negative bacteria.
- Source :
-
Journal of Bioscience & Bioengineering . Sep2015, Vol. 120 Issue 3, p335-339. 5p. - Publication Year :
- 2015
-
Abstract
- Our work here, for the first time, reported the antibacterial activity of kojic acid-grafted-chitosan oligosaccharides (COS/KA) against three gram-positive and three gram-negative bacteria. Integrity of cell membrane, outer membrane (OM) and inner membrane (IM) permeabilization assay, alkaline phosphatase (ALP) and glucose-6-phosphate dehydrogenase (G6PDH) assay, and SDS-PAGE assay techniques were used to investigate the interactions between COS/KA and bacterial membranes. The antibacterial activity of COS/KA was higher than those of unmodified COS. The electric conductivity of bacteria suspensions increased, followed by increasing of the units of average release for ALP and G6PDH. COS/KA can also rapidly increase the 1- N -phenylanphthylamine (NPN) uptake and the release of β-galactosidase via increasing the permeability of OM and IM in Escherichia coli . SDS-PAGE indicated the content of cellular soluble proteins decreased significantly in COS/KA-treated bacteria. Hence, COS/KA has potential in food industry and biomedical sciences. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13891723
- Volume :
- 120
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Journal of Bioscience & Bioengineering
- Publication Type :
- Academic Journal
- Accession number :
- 108679134
- Full Text :
- https://doi.org/10.1016/j.jbiosc.2015.01.010