Cloning and Expression Analysis of THWRKY12 Gene from Tamarix hispida.

[Objective] This study aimed to clone and analyze the expression of THWRKY12 gene in Tamarix hispida. [Method] T. hispida seedlings were treated with 400 mmol/L NaCl solution, 20% PEG and 100 µmol /L ABA, respectively. The expression of THWRKY12 gene in different tissues was investigated by RT-PCR technology. [Result] Under treatments of NaCl and PEG, the expression of THWRKY12 gene in different tissues of T. hispida seedlings showed an overall upregulated trend, suggesting that THWRKY12 is related with the saline-alkali resistance and drought resistance of T. hispida. Under ABA treatment, THWRKY12 gene had approximately the same expression pattern with the former two, indicating that THWRKY12 gene may involve in the regulation and control of the saline-alkali resistance and drought resistance of T. hispida through the signal pathway regulated by ABA. [Conclusion] This study laid foundation for investigating the functions of WRKY gene in the stress resistance of T. hispida. [ABSTRACT FROM AUTHOR]