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The role of plasma membrane H+- ATPase in jasmonate-induced ion fluxes and stomatal closure in Arabidopsis thaliana.
- Source :
-
Plant Journal . Aug2015, Vol. 83 Issue 4, p638-649. 12p. - Publication Year :
- 2015
-
Abstract
- Methyl jasmonate (Me JA) elicits stomatal closure in many plant species. Stomatal closure is accompanied by large ion fluxes across the plasma membrane ( PM). Here, we recorded the transmembrane ion fluxes of H+, Ca2+ and K+ in guard cells of wild-type (Col-0) Arabidopsis, the CORONATINE INSENSITIVE1 ( COI1) mutant coi1-1 and the PM H+- ATPase mutants aha1-6 and aha1-7, using a non-invasive micro-test technique. We showed that Me JA induced transmembrane H+ efflux, Ca2+ influx and K+ efflux across the PM of Col-0 guard cells. However, this ion transport was abolished in coi1-1 guard cells, suggesting that Me JA-induced transmembrane ion flux requires COI1. Furthermore, the H+ efflux and Ca2+ influx in Col-0 guard cells was impaired by vanadate pre-treatment or PM H+- ATPase mutation, suggesting that the rapid H+ efflux mediated by PM H+- ATPases could function upstream of the Ca2+ flux. After the rapid H+ efflux, the Col-0 guard cells had a longer oscillation period than before Me JA treatment, indicating that the activity of the PM H+- ATPase was reduced. Finally, the elevation of cytosolic Ca2+ concentration and the depolarized PM drive the efflux of K+ from the cell, resulting in loss of turgor and closure of the stomata. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09607412
- Volume :
- 83
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Plant Journal
- Publication Type :
- Academic Journal
- Accession number :
- 108790022
- Full Text :
- https://doi.org/10.1111/tpj.12915