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Andrographolide Enhances Proliferation and Prevents Dedifferentiation of Rabbit Articular Chondrocytes: An In Vitro Study.
- Source :
-
Evidence-based Complementary & Alternative Medicine (eCAM) . 2015, p1-10. 10p. - Publication Year :
- 2015
-
Abstract
- As the main active constituent of Andrographis paniculata that was applied in treatment of many diseases including inflammation in ancient China, andrographolide (ANDRO) was found to facilitate reduction of edema and analgesia in arthritis. This suggested that ANDRO may be promising anti-inflammatory agent to relieve destruction and degeneration of cartilage after inflammation. In this study, the effect of ANDRO on rabbit articular chondrocytes in vitro was investigated. Results showed that not more than 8f(M ANDRO did no harm to chondrocytes (P < 0.05). DNA content and glycosaminoglycan (GAG) /DNA were, respectively, improved in ANDRO groups comparing to the control (P < 0.05). ANDRO could promote expression of aggrecan, collagen II, and Sox9 genes while downregulating expression of collagen I gene (P < 0.05). Furthermore, hypertrophy that may result in chondrocyte ossification could not be detected in all groups (P > 0.05). The viability assay, hematoxylin-eosin, safranin O, and immunohistochemical staining also showed better performances in ANDRO groups. As to the doses, 3 µM ANDRO showed the best performance. The results indicate that ANDRO can accelerate proliferation of rabbit articular chondrocytes in vitro and meanwhile maintain the phenotype, which may provide valuable references for further exploration on arthritis. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DRUG therapy for arthritis
*INFLAMMATION prevention
*DNA analysis
*ANALYTICAL biochemistry
*ANIMAL experimentation
*BIOLOGICAL assay
*COLLECTION & preservation of biological specimens
*CARTILAGE cells
*CELL physiology
*CELL surface antigens
*COLLAGEN
*GENE expression
*GLYCOSAMINOGLYCANS
*HERBAL medicine
*IMMUNODIAGNOSIS
*IMMUNOHISTOCHEMISTRY
*CHINESE medicine
*PROBABILITY theory
*RABBITS
*RESEARCH funding
*STAINS & staining (Microscopy)
*DATA analysis software
*DESCRIPTIVE statistics
*IN vitro studies
*ONE-way analysis of variance
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Database :
- Academic Search Index
- Journal :
- Evidence-based Complementary & Alternative Medicine (eCAM)
- Publication Type :
- Academic Journal
- Accession number :
- 108824984
- Full Text :
- https://doi.org/10.1155/2015/984850