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Effector Phenotype of Plasmodium falciparum-Specific CD4+ T Cells Is Influenced by Both Age and Transmission Intensity in Naturally Exposed Populations.
- Source :
-
Journal of Infectious Diseases . 8/1/2015, Vol. 212 Issue 3, p416-425. 10p. - Publication Year :
- 2015
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Abstract
- Background. Mechanisms mediating immunity to malaria remain unclear, but animal data and experimental human vaccination models suggest a critical role for CD4+ T cells. Advances in multiparametric flow cytometry have revealed that the functional quality of pathogen-specific CD4+ T cells determines immune protection in many infectious models. Little is known about the functional characteristics of Plasmodium-specific CD4+ T-cell responses in immune and nonimmune individuals. Methods. We compared T-cell responses to Plasmodium falciparum among household-matched children and adults residing in settings of high or low malaria transmission in Uganda. Peripheral blood mononuclear cells were stimulated with P. falciparum antigen, and interferon γ (IFN-γ), interleukin 2, interleukin 10, and tumor necrosis factor α (TNF-α) production was analyzed via multiparametric flow cytometry. Results. We found that the magnitude of the CD4+ T-cell responses was greater in areas of high transmission but similar between children and adults in each setting type. In the high-transmission setting, most P. falciparum-specific CD4+ T-cells in children produced interleukin 10, while responses in adults were dominated by IFN-γ and TNF-α. In contrast, in the low-transmission setting, responses in both children and adults were dominated by IFN-γ and TNF-α. Conclusions. These findings highlight major differences in the CD4+ T-cell response of immune adults and nonimmune children that may be relevant for immune protection from malaria. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00221899
- Volume :
- 212
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Journal of Infectious Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 108911406
- Full Text :
- https://doi.org/10.1093/infdis/jiv054