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IL-6 essential for development of gut barrier dysfunction after hemorrhagic shock and resuscitation in mice.
- Source :
-
American Journal of Physiology: Gastrointestinal & Liver Physiology . Sep2003, Vol. 285 Issue 3, pG621-G629. 9p. 4 Color Photographs, 16 Graphs. - Publication Year :
- 2003
-
Abstract
- We sought to determine the role of IL-6 as a mediator of the alterations in gut barrier function that occur after hemorrhagic shock and resuscitation (HS/R). C57B1/6 wild-type (WT) and IL-6 knockout (KO) mice on a C57B1/6 background were subjected to either a sham procedure or HS/R. Organ and tissue samples were obtained 4 h after resuscitation. In WT mice, HS/R significantly increased ileal mucosal permeability to fluorescein isothiocyanate-1abeled dextran (average molecular mass, 4 kDa) and bacterial translocation to mesenteric lymph nodes. These alterations in gut barrier function were not observed in IL-6 KO animals. HS/R increased ileal steady-state mRNA levels for IL-6, TNF, and IL-10 in WT but not in IL-6 KO mice. Ileal mucosal expression of the tight junction protein, ZO-1, decreased after HS/R in WT but not IL-6 KO mice. Collectively, these data support the view that expression of IL-6 is essential for the development of gut barrier dysfunction after HS/R. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01931857
- Volume :
- 285
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- American Journal of Physiology: Gastrointestinal & Liver Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 10893521
- Full Text :
- https://doi.org/10.1152/ajpgi.00177.2003