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Modulation of osteoblast differentiation and bone mass by 5-HT2A receptor signaling in mice.
- Source :
-
European Journal of Pharmacology . Sep2015, Vol. 762, p150-157. 8p. - Publication Year :
- 2015
-
Abstract
- Recent studies reported that serotonin (5-hydroxytryptamine, 5-HT) may be an endogenous paracrine and/or autocrine factor that is used for intercellular communication in bone cells and between multiple organs regulating bone homeostasis. In the present study, we showed that the administration of MDL11939, a selective 5-HT 2A receptor antagonist, reduced bone mass in mice. The loss of bone mass in MDL11939-treated mice was associated with impaired bone formation in vivo , as demonstrated by the lower expression of osterix ( Osx ) and osteocalcin than that in vehicle-treated mice. On the other hand, no significant differences were observed in osteoclast numbers between MDL11939- and vehicle-treated mice. The pharmacological blockade of 5-HT 2A receptor signaling significantly decreased alkaline phosphatase activity in osteoblastic cells. In addition, the knockdown of the 5-HT 2A receptor by a siRNA treatment decreased Osx , but not Runx2 gene expression in MC3T3-E1 cells. These results suggest that 5-HT 2A receptor signaling mediated bone mass by regulating osteoblast differentiation. [ABSTRACT FROM AUTHOR]
- Subjects :
- *OSTEOBLASTS
*LABORATORY mice
*CONNECTIVE tissue cells
*BONES
*LABORATORY animals
Subjects
Details
- Language :
- English
- ISSN :
- 00142999
- Volume :
- 762
- Database :
- Academic Search Index
- Journal :
- European Journal of Pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 108941429
- Full Text :
- https://doi.org/10.1016/j.ejphar.2015.05.048