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Improving spectral resolution in biological solid-state NMR using phase-alternated rCW heteronuclear decoupling.
- Source :
-
Chemical Physics Letters . Aug2015, Vol. 635, p339-344. 6p. - Publication Year :
- 2015
-
Abstract
- The successful application of solid-state NMR spectroscopy for structural study of biological macromolecules requires high spectral resolution. In presence of abundant H 1 spins, the resolution of the prevailing C 13 or N 15 chemical shift encoding experiments critically depends on the availability of efficient and robust heteronuclear decoupling methods in addition to the use of high-field instrumentation and fast sample spinning. Robustness of the decoupling method towards alterations in amplitude/offset of radio frequency fields due to varying sample states is important to ensure recording of spectra with high resolution over long sampling periods for insensitive samples. Here, we present a phase-alternated refocused continuous-wave decoupling method offering better resolution, easier setup, and higher robustness than previous methods. Improved decoupling is in part ascribed to more efficient cancellation of the residual heteronuclear, H 1 – C 13 , dipolar coupling interactions which are induced by homonuclear, H 1 – H 1 , dipolar coupling interactions. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00092614
- Volume :
- 635
- Database :
- Academic Search Index
- Journal :
- Chemical Physics Letters
- Publication Type :
- Academic Journal
- Accession number :
- 108985208
- Full Text :
- https://doi.org/10.1016/j.cplett.2015.07.008