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Huangzhi Oral Liquid Prevents Arrhythmias by Upregulating Caspase-3 and Apoptosis Network Proteins in Myocardial Ischemia-Reperfusion Injury in Rats.
- Source :
-
Evidence-based Complementary & Alternative Medicine (eCAM) . 5/17/2015, Vol. 2015, p1-11. 11p. 4 Color Photographs, 1 Graph. - Publication Year :
- 2015
-
Abstract
- To study the effect of Huangzhi oral liquid (HZOL) on I/R after 2 h and 4 h and determine its regulatory function on caspase-3 and protein networks. 70 SD male rats were randomly divided into seven groups and established myocardial I/R injury model by ligating the left anterior descending coronary artery. Myocardial infarction model was defined by TTC staining and color of the heart. The levels of CK-MB, CTnI, C-RPL, SOD, and MDA were tested at 2 h and 4 h after reperfusion. HE staining and ultramicrostructural were used to observe the pathological changes. The apoptotic index (AI) of cardiomyocyte was marked by TUNEL. The expression levels of caspase-3, p53, fas, Bcl-2, and Bax were tested by immunohistochemistry and western blot. HZOL corrected arrhythmia, improved the pathologic abnormalities, decreased CK-MB, CTnI, C-RPL, MDA, AI, caspase-3, p53, fas, and Bax, and increased SOD ans Bcl-2 with different times of myocardial reperfusion; this result was similar to the ISMOC (P>0.05). HZOL could inhibit arrhythmia at 2 and 4 h after I/R and ameliorate cardiac function, which was more significant at 4 h after reperfusion. This result may be related to decreased expression of caspase-3, p53, and fas and increased Bcl-2/Bax ratio. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ARRHYTHMIA prevention
*ANIMAL experimentation
*APOPTOSIS
*GENE expression
*HEART cells
*HERBAL medicine
*IMMUNOHISTOCHEMISTRY
*ISCHEMIA
*CHINESE medicine
*CARDIOMYOPATHIES
*MYOCARDIUM
*ORAL drug administration
*PROTEINS
*RATS
*REPERFUSION
*STAINS & staining (Microscopy)
*CONTROL groups
*WOUNDS & injuries
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Volume :
- 2015
- Database :
- Academic Search Index
- Journal :
- Evidence-based Complementary & Alternative Medicine (eCAM)
- Publication Type :
- Academic Journal
- Accession number :
- 109051148
- Full Text :
- https://doi.org/10.1155/2015/518926