Back to Search
Start Over
Residue depletion of ampicillin in eggs.
- Source :
-
Journal of Veterinary Pharmacology & Therapeutics . Oct2015, Vol. 38 Issue 5, p508-512. 5p. - Publication Year :
- 2015
-
Abstract
- A residue depletion study of ampicillin ( AMP) was performed after oral dosing (60.0 mg/kg and 120.0 mg/kg body weight once a day for 5 days) to laying hens, through the use of reversed-phase high-performance liquid chromatography with fluorescence detection ( RP- HPLC- FLD) to achieve detection of ampicillin residue in eggs. Limit of detection was 0.5 ng/g, and limit of quantitation was 1.2 ng/g for ampicillin. Extraction recoveries of ampicillin from samples fortified at 5.0-125.0 ng/g levels ranged from 77.5% to 84.6% in albumen, 77.9% to 87.5% in yolk, and 77.9% to 88.6% in whole egg, with coefficients of variation ≤9.3%. The maximum concentrations of ampicillin in albumen, yolk, and whole egg were detected at 1, 2, and 1 day after the last administration of ampicillin, respectively. Ampicillin was not detectable in albumen at day 9 of withdrawal time, at day 10 and 11 in yolk, and day 9 and 11 in whole egg at each of those 2 dose levels. The theoretical withdrawal time of AMP in whole egg was 6.730 and 7.296 days for 60 and 120 mg/kg oral dosage, respectively. This method also proved to be suitable as a rapid and reliable method for the determination of ampicillin in other poultry eggs. [ABSTRACT FROM AUTHOR]
- Subjects :
- *AMPICILLIN
*PENICILLIN G
*EGG substitutes
*ANIMAL clutches
*FOOD of animal origin
Subjects
Details
- Language :
- English
- ISSN :
- 01407783
- Volume :
- 38
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Journal of Veterinary Pharmacology & Therapeutics
- Publication Type :
- Academic Journal
- Accession number :
- 109227742
- Full Text :
- https://doi.org/10.1111/jvp.12211