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In Vitro Differentiation Potential of Human Placenta Derived Cells into Skin Cells.
- Source :
-
Stem Cells International . 7/1/2015, Vol. 2015, p1-11. 11p. - Publication Year :
- 2015
-
Abstract
- Skin autografting is the most viable and aesthetic technique for treatment of extensive burns; however, this practice has potential limitations. Harvesting cells from neonatal sources (such as placental tissue) is a simple, inexpensive, and noninvasive procedure. In the current study authors sought to evaluate in vitro potential of human placenta derived stem cells to develop into skin-like cells. After extensive washing, amniotic membrane and umbilical cord tissue were separated to harvest amniotic epithelial cells (AECs) and umbilical cord mesenchymal stem cells (UC-MSCs), respectively. Both types of cells were characterized for the expression of embryonic lineage markers and their growth characteristics were determined. AECs and UC-MSCs were induced to differentiate into keratinocytes-like and dermal fibroblasts-like cells, respectively. After induction, morphological changes were detected by microscopy. The differentiation potential was further assessed using immunostaining and RT-PCR analyses. AECs were positive for cytokeratins and E-Cadherin while UC-MSCs were positive for fibroblast specific makers. AECs differentiated into keratinocytes-like cells showed positive expression of keratinocyte specific cytokeratins, involucrin, and loricrin. UC-MSCs differentiated into dermal fibroblast-like cells indicated expression of collagen type 3, desmin, FGF-7, fibroblast activation protein alpha, procollagen-1, and vimentin. In conclusion, placenta is a potential source of cells to develop into skin-like cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PLACENTA
*SKIN
*EMBRYOLOGY
*BODY covering (Anatomy)
*CELLS
Subjects
Details
- Language :
- English
- ISSN :
- 1687966X
- Volume :
- 2015
- Database :
- Academic Search Index
- Journal :
- Stem Cells International
- Publication Type :
- Academic Journal
- Accession number :
- 109249271
- Full Text :
- https://doi.org/10.1155/2015/841062