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Immunogenicity of Newcastle disease virus vectors expressing Norwalk virus capsid protein in the presence or absence of VP2 protein.
- Source :
-
Virology . Oct2015, Vol. 484, p163-169. 7p. - Publication Year :
- 2015
-
Abstract
- Noroviruses are the most common cause of acute gastroenteritis in humans. Development of an effective vaccine is required for reducing their outbreaks. In order to develop a GI norovirus vaccine, Newcastle disease virus vectors, rLaSota and modified rBC, were used to express VP1 protein of Norwalk virus. Co-expression of VP1 and VP2 proteins by Newcastle disease virus vectors resulted in enhanced expression of Norwalk virus VP1 protein and self-assembly of VP1 protein into virus-like particles. Furthermore, the Norwalk virus-specific IgG response induced in mice by Newcastle disease virus vectors was similar to that induced by baculovirus-expressed virus-like particles in mice. However, the modified rBC vector in the presence of VP2 protein induced significantly higher levels of cellular and mucosal immune responses than those induced by baculovirus-expressed VLPs. These results indicate that Newcastle disease virus has great potential for developing a live Norwalk virus vaccine by inducing humoral, cellular and mucosal immune responses in humans. [ABSTRACT FROM AUTHOR]
- Subjects :
- *NEWCASTLE disease virus
*GENETIC vectors
*NOROVIRUSES
*CAPSIDS
*VIRAL proteins
Subjects
Details
- Language :
- English
- ISSN :
- 00426822
- Volume :
- 484
- Database :
- Academic Search Index
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 109258951
- Full Text :
- https://doi.org/10.1016/j.virol.2015.06.003