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TLR4/NF- κ B-Responsive MicroRNAs and Their Potential Target Genes: A Mouse Model of Skeletal Muscle Ischemia-Reperfusion Injury.
- Source :
-
BioMed Research International . 1/26/2015, Vol. 2015, p1-11. 11p. - Publication Year :
- 2015
-
Abstract
- Background. The aim of this study was to profile TLR4/NF-κB-responsive microRNAs (miRNAs) and their potential target genes in the skeletal muscles of mice following ischemia-reperfusion injury. Methods. Thigh skeletal muscles of C57BL/6, Tlr4−/−, and NF-κB−/− mice isolated based on femoral artery perfusion were subjected to ischemia for 2 h and reperfusion for 0 h, 4 h, 1 d, and 7 d. The muscle specimens were analyzed with miRNA arrays. Immunoprecipitation with an argonaute 2- (Ago2-) specific monoclonal antibody followed by whole genome microarray was performed to identify mRNA associated with the RNA-silencing machinery. The potential targets of each upregulated miRNA were identified by combined analysis involving the bioinformatics algorithm miRanda and whole genome expression. Results. Three TLR4/NF-κB-responsive miRNAs (miR-15a, miR-744, and miR-1196) were significantly upregulated in the muscles following ischemia-reperfusion injury. The combined in silico and whole genome microarray approaches identified 5, 4, and 20 potential target genes for miR-15a, miR-744, and miR-1196, respectively. Among the 3 genes (Zbed4, Lrsam1, and Ddx21) regulated by at least 2 of the 3 upregulated miRNAs, Lrsam1 and Ddx21 are known to be associated with the innate immunity pathway. Conclusions. This study profiled TLR4/NF-κB-responsive miRNAs and their potential target genes in mouse skeletal muscle subjected to ischemia-reperfusion injury. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CELL receptors
*ALGORITHMS
*ANIMAL experimentation
*BIOLOGICAL models
*CLUSTER analysis (Statistics)
*GENE expression
*GENOMES
*IMMUNOBLOTTING
*MICE
*PROBABILITY theory
*REPERFUSION injury
*RESEARCH funding
*RNA
*SPECTROPHOTOMETRY
*DNA-binding proteins
*BIOINFORMATICS
*OLIGONUCLEOTIDE arrays
*SKELETAL muscle
*GENE expression profiling
*PRECIPITIN tests
*EPIGENOMICS
*CELL physiology
Subjects
Details
- Language :
- English
- ISSN :
- 23146133
- Volume :
- 2015
- Database :
- Academic Search Index
- Journal :
- BioMed Research International
- Publication Type :
- Academic Journal
- Accession number :
- 109273183
- Full Text :
- https://doi.org/10.1155/2015/410721