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PCR for the Specific Detection of an Escherichia coli O157:H7 Laboratory Control Strain.
- Source :
-
Journal of Food Protection . Sep2015, Vol. 78 Issue 9, p1738-1744. 7p. - Publication Year :
- 2015
-
Abstract
- Control strains of bacterial pathogens such as Escherichia coli O157:H7 are commonly processed in parallel with test samples in food microbiology laboratories as a quality control measure to assure the satisfactory performance of materials used in the analytical procedure. Before positive findings can be reported for risk management purposes, analysts must have a means of verifying that pathogenic bacteria (e.g., E. coli O157:H7) recovered from test samples are not due to inadvertent contamination with the control strain routinely handled in the laboratory environment. Here, we report on the application of an in-house bioinformatic pipeline for the identification of unique genomic signature sequences in the development of specific oligonucleotide primers enabling the identification of a common positive control strain, E. coli O157:H7 (ATCC 35150), using a simple PCR procedure. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0362028X
- Volume :
- 78
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Journal of Food Protection
- Publication Type :
- Academic Journal
- Accession number :
- 109333676
- Full Text :
- https://doi.org/10.4315/0362-028X.JFP-15-147