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Functional analysis of the Arabidopsis thaliana CHLOROPLAST BIOGENESIS 19 pentatricopeptide repeat editing protein.
- Source :
-
New Phytologist . Oct2015, Vol. 208 Issue 2, p430-441. 12p. 1 Color Photograph, 6 Diagrams, 1 Chart. - Publication Year :
- 2015
-
Abstract
- The Arabidopsis thaliana pentatricopeptide repeat ( PPR) family of proteins contains several degenerate 35-aa motifs named PPR repeats. These proteins control diverse post-transcriptional regulatory mechanisms, including RNA editing. CLB19 belongs to the PLS subfamily of PPR proteins and is essential for the editing and functionality of the subunit A of plastid-encoded RNA polymerase (RpoA) and the catalytic subunit of the Clp protease (ClpP1)., We demonstrate in vitro that CLB19 has a specific interaction with these two targets, in spite of their modest sequence similarity. Using site-directed mutagenesis of the rpoA target, we analyzed the essential nucleotides required for CLB19- rpoA interactions., We verified that, similar to other editing proteins, the C-terminal E domain of CLB19 is essential for editing but not for RNA binding. Using biomolecular fluorescence complementation, we demonstrated that the E domain of CLB19 interacts with the RNA-interacting protein MORF2/ RIP2 but not with MORF9/ RIP9. An interesting finding from this analysis was that overexpression of a truncated CLB19 protein lacking the E domain interferes with cell fate during megasporogenesis and the subsequent establishment of a female gametophyte, supporting an important role of plastids in female gametogenesis., Together these analyses provide important clues about the particularities of the CLB19 editing protein. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0028646X
- Volume :
- 208
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- New Phytologist
- Publication Type :
- Academic Journal
- Accession number :
- 109509745
- Full Text :
- https://doi.org/10.1111/nph.13468