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Genome-wide Maps of Nuclear Lamina Interactions in Single Human Cells.
- Source :
-
Cell . Sep2015, Vol. 163 Issue 1, p134-147. 14p. - Publication Year :
- 2015
-
Abstract
- Summary Mammalian interphase chromosomes interact with the nuclear lamina (NL) through hundreds of large lamina-associated domains (LADs). We report a method to map NL contacts genome-wide in single human cells. Analysis of nearly 400 maps reveals a core architecture consisting of gene-poor LADs that contact the NL with high cell-to-cell consistency, interspersed by LADs with more variable NL interactions. The variable contacts tend to be cell-type specific and are more sensitive to changes in genome ploidy than the consistent contacts. Single-cell maps indicate that NL contacts involve multivalent interactions over hundreds of kilobases. Moreover, we observe extensive intra-chromosomal coordination of NL contacts, even over tens of megabases. Such coordinated loci exhibit preferential interactions as detected by Hi-C. Finally, the consistency of NL contacts is inversely linked to gene activity in single cells and correlates positively with the heterochromatic histone modification H3K9me3. These results highlight fundamental principles of single-cell chromatin organization. Video Abstract [ABSTRACT FROM AUTHOR]
- Subjects :
- *CELL communication
*INTERPHASE
*GENOMES
*CHROMATIN
*CHROMOSOMES
Subjects
Details
- Language :
- English
- ISSN :
- 00928674
- Volume :
- 163
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Cell
- Publication Type :
- Academic Journal
- Accession number :
- 109568333
- Full Text :
- https://doi.org/10.1016/j.cell.2015.08.040