A new spermidine macrocyclic alkaloid isolated from Gymnosporia arenicola leaf.

The isolation and structural elucidation of a macrocyclic alkaloid, characterized by the presence of a 13-membered macrolactam ring containing a spermidine unit N -linked to a benzoyl group is hereby reported. The structure of this previously unknown spermidine alkaloid isolated from Gymnosporia arenicola ( Celastraceae ) leaves has been elucidated by 1 H and 13 C NMR spectroscopy (including bidimensional analysis) and further characterized by high-resolution mass spectrometry and polarimetry. A route for the biosynthesis of this new bioactive macrocycle is proposed and the cytotoxicity of the compound was evaluated against two ATCC cell lines – one normal-derived (MCF10A) and one cancer-derived cell line (MCF7) – using the MTT assay. The alkaloid revealed to be non-cytotoxic against both cell lines. The IC 50 values from the cells were also determined. [ABSTRACT FROM AUTHOR]