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Alloantigen-specific T-cell hyporesponsiveness induced by dnIKK2 gene-transfected recipient immature dendritic cells.
- Source :
-
Cellular Immunology . Oct2015, Vol. 297 Issue 2, p100-107. 8p. - Publication Year :
- 2015
-
Abstract
- Immature dendritic cells (iDCs) have been shown to be able to induce peripheral T-cell tolerance through distinct pathways. Here, we investigated the tolerogenic property of recipient iDCs whose maturation was arrested by a dominant negative mutant of inhibitor of nuclear factor kappa-B kinase 2 (dnIKK2) gene. We found that dnIKK2-iDCs presented a typical semi-mature morphology and expressed lower levels of CD80 and CD86, slightly higher MHC-II than untransfected iDCs. The expression of these molecules had no significant change even dnIKK2-iDCs were pulsed by donor antigen. In primary mixed leukocyte reaction (MLR), dnIKK2-iDCs exhibited impaired ability to stimulate allogeneic T-cells, but induced CD4 + CD25 − T-cell formation. In co-culture MLR, these CD4 + CD25 − T-cells suppressed T-cell alloreaction in an antigen-specific manner. Besides, CD4 + CD25 − T-cells inhibited IL-2 and IFN-γ release, whereas promoted IL-10 and TGF-β secretion. These data suggested recipient dnIKK2-iDCs could maintain peripheral tolerance through down-regulating costimulatory molecule expressions and inducing CD4 + CD25 − T-cell formation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00088749
- Volume :
- 297
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Cellular Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 110409938
- Full Text :
- https://doi.org/10.1016/j.cellimm.2015.07.004