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mi-134 Modulates the Proliferation of Human Cardiomyocyte Progenitor Cells by Targeting Meis2.
- Source :
-
International Journal of Molecular Sciences . 2015, Vol. 16 Issue 10, p25199-25213. 19p. - Publication Year :
- 2015
-
Abstract
- Cardiomyocyte progenitor cells play essential roles in early heart development, which requires highly controlled cellular organization. microRNAs (miRs) are involved in various cell behaviors by post-transcriptional regulation of target genes. However, the roles of miRNAs in human cardiomyocyte progenitor cells (hCMPCs) remain to be elucidated. Our previous study showed that mi-134 was significantly downregulated in heart tissue suffering from congenital heart disease, underlying the potential role of mi-134 in cardiogenesis. In the present work, we showed that the upregulation of mi-134 reduced the proliferation of hCMPCs, as determined by EdU assay and Ki-67 immunostaining, while the inhibition of mi-134 exhibited an opposite effect. Both up- and downregulation of mi-134 expression altered the transcriptional level of cell-cycle genes. We identified Meis2 as the target of mi-134 in the regulation of hCMPC proliferation through bioinformatic prediction, luciferase reporter assay and western blot. The over-expression of Meis2 mitigated the effect of mi-134 on hCMPC proliferation. Moreover, mi-134 did not change the degree of hCMPC differentiation into cardiomyocytes in our model, suggesting that mi-134 is not required in this process. These findings reveal an essential role for mi-134 in cardiomyocyte progenitor cell biology and provide new insights into the physiology and pathology of cardiogenesis. [ABSTRACT FROM AUTHOR]
- Subjects :
- *HEART cells
*CELL proliferation
*MICRORNA
*HEART development
*PROGENITOR cells
Subjects
Details
- Language :
- English
- ISSN :
- 16616596
- Volume :
- 16
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- International Journal of Molecular Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 110644493
- Full Text :
- https://doi.org/10.3390/ijms161025199