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Higher proliferation of peritumoral endothelial cells to IL-6/sIL-6R than tumoral endothelial cells in hepatocellular carcinoma.
- Source :
-
BMC Cancer . 11/2/2015, Vol. 15, p1-9. 9p. 1 Chart, 4 Graphs. - Publication Year :
- 2015
-
Abstract
- <bold>Background: </bold>This study aimed to explore the responses to the interleukin-6 (IL-6)/soluble interleukin-6 receptor (sIL-6R) complex in peritumoral endothelial cells (PECs) and tumor endothelial cells (TECs), as well as determine the signaling pathways in the angiogenesis of hepatocellular carcinoma (HCC).<bold>Methods: </bold>The expression of IL-6, IL-6R, gp130, CD68, HIF-1α, and microvessel density (MVD) were assessed with an orthotopic xenograft model in nude mice. ECs were incubated under hypoxic conditions to detect IL-6 and gp130. The proliferation of PECs and TECs in the presence of IL-6 and sIL-6R, as well as the expression of gp130, JAK2/STAT3, PI3K/AKT in endothelial cells were measured.<bold>Results: </bold>Peritumoral IL-6, IL-6R, gp130, CD68, and HIF-1α expression, as well as MVD, gradually increased during tumor growth. Hypoxia could directly induce IL-6 expression, but not gp130 in PECs. The co-culture of IL-6/sIL-6R induced much higher PEC proliferation and gp130 expression, as well as the elevated phosphorylation of JAK2 and STAT3, however not the phosphorylation of PI3K and AKT.<bold>Conclusions: </bold>PECs exhibited higher proliferation in response to IL-6/sIL-6R co-treatment compared with TECs in HCC via the up-regulation of gp130 /JAK2/STAT3. PEC and its associated peritumoral angiogenesis microenvironment may be a potential novel target for anti-angiogenic treatment. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ENDOTHELIAL cells
*INTERLEUKIN-6
*LIVER cancer
*XENOGRAFTS
*LABORATORY mice
*GENE expression
*PHOSPHORYLATION
*CELL proliferation
*ANIMAL experimentation
*CARRIER proteins
*CELL lines
*CELL physiology
*CELLULAR signal transduction
*EPITHELIAL cells
*GENES
*HEPATOCELLULAR carcinoma
*INTERLEUKINS
*LIVER tumors
*RESEARCH methodology
*MICE
*PHOSPHOTRANSFERASES
*PROTEINS
*TISSUE culture
*PATHOLOGIC neovascularization
Subjects
Details
- Language :
- English
- ISSN :
- 14712407
- Volume :
- 15
- Database :
- Academic Search Index
- Journal :
- BMC Cancer
- Publication Type :
- Academic Journal
- Accession number :
- 110710440
- Full Text :
- https://doi.org/10.1186/s12885-015-1763-2