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Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of SET/TAF-IβΔN from Homo sapiens.
- Source :
-
Acta Crystallographica: Section F (Wiley-Blackwell) . Aug2010, Vol. 66 Issue 8, p926-928. 3p. - Publication Year :
- 2010
-
Abstract
- The histone chaperone SET encoded by the SET gene, which is also known as template-activating factor Iβ (TAF-Iβ), is a multifunctional molecule that is involved in many biological phenomena such as histone binding, nucleosome assembly, chromatin remodelling, replication, transcription and apoptosis. A truncated SET/TAF-IβΔN protein that lacked the first 22 residues of the N-terminus but contained the C-terminal acidic domain and an additional His6 tag at the C-terminus was overexpressed in Escherichia coli and crystallized by the hanging-drop vapour-diffusion method using sodium acetate as precipitant at 283 K. The crystals diffracted to 2.7 Å resolution and belonged to space group P43212. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CHROMATIN
*HISTONES
*ESCHERICHIA coli
*CRYSTALLIZATION
*X-ray crystallography
Subjects
Details
- Language :
- English
- ISSN :
- 17443091
- Volume :
- 66
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Acta Crystallographica: Section F (Wiley-Blackwell)
- Publication Type :
- Academic Journal
- Accession number :
- 110812490
- Full Text :
- https://doi.org/10.1107/S1744309110021779