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Shikonin inhibits adipogenic differentiation via regulation of mir-34a-FKBP1B.
- Source :
-
Biochemical & Biophysical Research Communications . Nov2015, Vol. 467 Issue 4, p941-947. 7p. - Publication Year :
- 2015
-
Abstract
- Shikonin is a naturally occurring naphthoquinone pigment and a major constituent present in Lithospermum erythrorhizon . Since microRNAs (miRNAs) are one of the key post-transcriptional regulators of adipogenesis, their manipulation represents a potential new strategy to inhibit adipogenesis. Our aim was to investigate shikonin-dependent inhibition of adipogenesis with an emphasis on miRNA-related processes. Mir-34a increased during induced adipogenesis, and this was suppressed in the presence of shikonin. mRNA expression of FKBP1B, a suggested target of mir-34a according to bioinformatics studies, decreased during adipogenesis, but was recovered by shikonin treatment, which reversely correlated with mir-34a expression. A mir-34a inhibitor suppressed MDI-induced adipogenesis by blocking PPARγ and C/EBPα expression, while suppression of mir-34a recovered MDI-induced down-regulation of FKBP1B expression. A mir-34a mimic decreased FKBP1B mRNA expression in 3T3-L1 preadipocytes. We also observed that mir-34a bound directly to the 3′-untranslated region of FKBP1B. Finally, FKBP1B overexpression attenuated MDI-induced adipogenesis, PPARγ, and C/EBPα expression. These results suggest that mir-34a regulates adipogenesis by targeting FKBP1B expression. Our findings reveal that shikonin prevents adipogenesis by blocking the mir-34a-FKBP1B pathway which represents a promising potential target for preventing obesity. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SHIKONIN
*ADIPOGENESIS
*MICRORNA
*GENETIC regulation
*NAPHTHOQUINONE
*LITHOSPERMUM
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 467
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 110853992
- Full Text :
- https://doi.org/10.1016/j.bbrc.2015.10.039