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Development of a high yield expression and purification system for Domain I of Beta-2-glycoprotein I for the treatment of APS.
- Source :
-
BMC Biotechnology . 11/14/2015, Vol. 15, p1-11. 11p. - Publication Year :
- 2015
-
Abstract
- Background: In this paper we describe a novel method to achieve high yield bacterial expression of a small protein domain with considerable therapeutic potential; Domain I of Beta-2-glycoprotein I (β2GPI). β2GPI is intrinsic to the pathological progression of the Antiphospholipid Syndrome (APS). Patients develop autoantibodies targeting an epitope located on the N-terminal Domain I of β2GPI rendering this domain of interest as a possible therapeutic. Results: This new method of production of Domain I of β2GPI has increased the production yield by ~20 fold compared to previous methods in E.coli. This largely scalable, partially automated method produces 50-75 mg of pure, folded, active Domain I of β2GPI per litre of expression media. Conclusion: The application of this method may enable production of Domain I on sufficient scale to allow its use as a therapeutic. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14726750
- Volume :
- 15
- Database :
- Academic Search Index
- Journal :
- BMC Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 111009463
- Full Text :
- https://doi.org/10.1186/s12896-015-0222-0