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Construction and immunological evaluation of recombinant Lactobacillus plantarum expressing HN of Newcastle disease virus and DC- targeting peptide fusion protein.

Authors :
Jiang, Yanlong
Hu, Jingtao
Guo, Yanbing
Yang, Wentao
Ye, Liping
Shi, Chunwei
Liu, Yuying
Yang, Guilian
Wang, Chunfeng
Source :
Journal of Biotechnology. Dec2015, Vol. 216, p82-89. 8p.
Publication Year :
2015

Abstract

Newcastle disease virus (NDV) has been considered as one of the most severe threats to poultry industry. In this study, we constructed a series of food grade recombinant Lactobacillus plantarum ( L. plantarum ) strains (RLP) synthesizing either virus hemagglutinin–neuraminidase protein (HN) alone or HN fused with DC cells targeting peptides (DCpep), named RLP(pSIP409-HN) and RLP (pSIP409-HN-DCpep), respectively. The immune responses and protective efficacy were then evaluated in chickens. Results showed that the presence of DCpep in RLP (pSIP409-HN-DCpep) group significantly increased the production of secretory immunoglobulin A (SIgA) in intestines and the percentages of CD3 + CD4 + T cells in spleen and peripheral blood leukocytes ( P < 0.05) compared to chickens immunized with RLP (pSIP409-HN). In addition, the similar enhancement effects were also observed with regard to trachea SIgA, T lymphocytes proliferation and survival rates after NDV challenge, even without significant statics differences. The results demonstrated the possibility to take use of DCpep as an immune adjuvant in the design of NDV vaccine. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01681656
Volume :
216
Database :
Academic Search Index
Journal :
Journal of Biotechnology
Publication Type :
Academic Journal
Accession number :
111292540
Full Text :
https://doi.org/10.1016/j.jbiotec.2015.09.033