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Shenfu injection protects human ECV304 cells from hydrogen peroxide via its anti-apoptosis way.
- Source :
-
Journal of Ethnopharmacology . Apr2015, Vol. 163, p203-209. 7p. - Publication Year :
- 2015
-
Abstract
- Ethnopharmacological relevance The pathogenesis of thromboangiitis obliterans (TAO) has not been fully elucidated until now. Shenfu injection (SFI), a traditional Chinese formula has been widely used clinically for the treatment of cardiovascular diseases for more than two decade. Our previous results first suggested that SFI can cause a significant therapeutic effect on experimental TAO model rats. This experiment was designed to further investigate the protective effect of SFI on VEC damaged by hydrogen peroxide (H 2 O 2 ) oxidative stress in vitro . Meterials and methods The cell viability was evaluated by the MTT assay, the activities of SOD and GSH-PX and the content of MDA in the supernatants of the cultured ECV304 cells were evaluated by a colorimetry method, cell apoptosis was detected by flow cytometry and an AO/EB double staining method. The protein expressions of Bcl2, Bax and caspase-3 were examined by Western blotting. Results When compared with control group, lower survival rate of ECV304 cells was observed in H 2 O 2 group ( p <0.01) ; 20 μl/ml, 30 μl/ml and 40 μl/ml SFI increased the survival rate of ECV304 cells under H 2 O 2 oxidative stress ( p <0.05 and p <0.01). The activities of SOD and GSH-PX were higher and MDA level was lower in H 2 O 2 group than those in control group. These effects of H 2 O 2 on SOD, GSH-PX activities and MDA content were reversed by SFI in concentration-dependent way ( p <0.05 and p <0.01). Flow cytometry and AO–EB double staining discovered that SFI pretreatment inhibited the ECV304 cells apoptosis. The protein expression of caspase3 in 30 μl/ml and 40 μl/ml SFI groups significantly decreased whereas Bcl2 protein expressions in 20 μl/ml, 30 μl/ml and 40 μl/ml SFI groups were higher than H 2 O 2 group, with Bax protein expression much lower than H 2 O 2 group ( p <0.05 and p <0.01). Conclusions Our findings suggest that SFI could prevent the ECV304 cells against H 2 O 2 oxidative-stress by enhancing antioxidant enzyme activities, reducing the membrane lipid peroxidation, as well as upregulating antiapoptotic and downregulating apoptosis protein expressions. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ANIMAL experimentation
*ANTIOXIDANTS
*APOPTOSIS
*BIOLOGICAL assay
*EPITHELIAL cells
*EXPERIMENTAL design
*FLOW cytometry
*GENE expression
*HERBAL medicine
*HYDROGEN peroxide
*CHINESE medicine
*LIPID peroxidation (Biology)
*OXIDOREDUCTASES
*RATS
*WESTERN immunoblotting
*OXIDATIVE stress
*IN vitro studies
Subjects
Details
- Language :
- English
- ISSN :
- 03788741
- Volume :
- 163
- Database :
- Academic Search Index
- Journal :
- Journal of Ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 111292672
- Full Text :
- https://doi.org/10.1016/j.jep.2015.01.032