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Expression and purification of Src I from sea anemone Sagartia rosea as a recombinant non-fusion protein
- Source :
-
Protein Expression & Purification . Nov2003, Vol. 32 Issue 1, p161. 6p. - Publication Year :
- 2003
-
Abstract
- The cDNA coding for an acidic actinoporin, Sagartia rosea cytolysin I (Src I), has been isolated, cloned into pGEM-T Easy Vector, and sequenced. The region encoding matured Src I was also cloned into prokaryotic expression vector pBV220 and expressed in Escherichia coli as a non-fusion protein in the form of inclusion body. Through washing and denaturation–renaturation step, the recombinant Src I was purified by Q Sepharose Fast Flow ion exchange chromatography and Phenyl Sepharose hydrophobic interaction chromatography. The two-step purification of Src I was effective, simple, and suitable for isolating large amount of high purity (above 95%) recombinant Src I. [Copyright &y& Elsevier]
- Subjects :
- *DNA
*CLONING
*PROKARYOTES
*GENE expression
Subjects
Details
- Language :
- English
- ISSN :
- 10465928
- Volume :
- 32
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Protein Expression & Purification
- Publication Type :
- Academic Journal
- Accession number :
- 11172527
- Full Text :
- https://doi.org/10.1016/S1046-5928(03)00229-8