Rapid detection of ochratoxin A on membrane by dot immunogold filtration assay.

BACKGROUND Ochratoxin A ( OTA), a widely distributed mycotoxin produced by certain species of Aspergillus and Penicillium, has been identified as a carcinogenic, hepatotoxic, teratogenic, nephrotoxic and immunotoxic toxin. To reduce the risk of OTA contamination, a rapid, inexpensive, suitable and on-site assay for its detection is required. RESULTS In this study a dot immunogold filtration assay ( DIGFA) of OTA on high-flow nitrocellulose membrane was developed. Firstly colloidal gold was synthesized and colloidal gold-polyclonal antibody ( PcAb) conjugates against OTA were prepared at the optimal colloidal gold-labeled pH value and package amount. Then the colloidal gold- PcAb conjugates were used to develop the OTA DIGFA. The results demonstrated a visual detection limit of approximately 10 ng mL−1 OTA. In addition, this method had no cross-reaction with zearalenone, aflatoxin B1 or citrinin. CONCLUSION These results indicated that the developed DIGFA could be applied for the actual detection of samples without complicated steps. © 2015 Society of Chemical Industry [ABSTRACT FROM AUTHOR]